Journal: Frontiers in Medicine

Article Title: Gene Expression in Amnion-Derived Cells Cultured on Recombinant Laminin 332—A Preliminary Study

doi: 10.3389/fmed.2021.719899

Figure Lengend Snippet: Antibodies and isotype controls used for immunodetection of integrin subunits and CK-7.

Article Snippet: ITGB4 , Anti-integrin beta 4 antibody, clone 439-9B (Novus Biologicals) , Alexa Fluor 488 Mouse monoclonal Anti-Rat IgG2b heavy chain (Abcam) , Rat IgG2b Isotype Control (Novus Biologicals).

Techniques: Immunodetection, Control, Purification, Plasmid Preparation

Journal: Blood Cancer Journal

Article Title: A superantigen-based MHC class II-targeted cancer immunotherapy for the treatment of acute myeloid leukemia

doi: 10.1038/s41408-025-01391-w

Figure Lengend Snippet: A Flow cytometry of various AML cell lines showing CD33 cell surface expression with median fluorescence intensity (MFI) quantification. Significance was determined by Mann-Whitney test, ** P < 0.01. B Western blot for anti-6X His tagged recombinant proteins. C ELISA for M2T-CD33 binding to various MHCII alleles, including human, mouse, and dog. IgG1 Fc Knob-in-Hole as a control for MHCII alleles constructed with IgG1 Fc stalk. BSA as negative control. D Flow cytometry of Daudi and Jurkat cells showing TCR and MHCII (HLA-DR) expression, M2T-CD33 binding, and M2T-CD33 double mutant (DM) binding. E Flow cytometry of human PBMCs (healthy donor #1) gating on CD3 or CD19 and then comparing binding of CD33 vs. M2T-CD33. F Flow cytometry of mouse splenocytes gating on CD3 or CD19 and then comparing binding of CD33 vs. M2T-CD33.

Article Snippet: His-Tag Rabbit mAb AF488 (Cat #14930S), Anti-Human CD3 PE (Cat #46233S), and Rat IgG2b Isotype Control PE (Cat #27426S) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Flow Cytometry, Expressing, Fluorescence, MANN-WHITNEY, Western Blot, Recombinant, Enzyme-linked Immunosorbent Assay, Binding Assay, Control, Construct, Negative Control, Mutagenesis

Journal: Blood Cancer Journal

Article Title: A superantigen-based MHC class II-targeted cancer immunotherapy for the treatment of acute myeloid leukemia

doi: 10.1038/s41408-025-01391-w

Figure Lengend Snippet: A Flow cytometry of CD33 expression on C1498-hCD33 cells prior to injection. Representative survival of the model on right. B ELISA of anti-CD33 IgG over time after injection with M2T-CD33 or controls. Proteins were injected at a dose of 5 µg per mouse as indicated by triangles. C , D Mice were injected with 5 µg M2T-CD33 or controls as indicated by triangles and then challenged 1 week later with 0.5 × 10 6 C1498-hCD33 cells (Day 0; N = 10). C ELISA of anti-CD33 IgG 1 week after third M2T-CD33 dose. Error bars represent standard error of the mean (SEM). Significance determined by Kruskal-Wallis test and Dunn’s multiple comparisons test, **** P < 0.0001. D Kaplan-Meier survival curves. Significance was determined by log-rank Mantel-Cox test, compared to vehicle control. E Mice were injected with 5 µg M2T-CD33 or controls as indicated by triangles and then challenged 2 weeks later with 0.5 × 10 6 C1498-hCD33 cells (Day 0). The therapeutic regimen was then repeated every 4 weeks ( N = 9–10). Kaplan–Meier survival curves shown. Survival significance was determined by log-rank Mantel-Cox test, compared to control.

Article Snippet: His-Tag Rabbit mAb AF488 (Cat #14930S), Anti-Human CD3 PE (Cat #46233S), and Rat IgG2b Isotype Control PE (Cat #27426S) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Flow Cytometry, Expressing, Injection, Enzyme-linked Immunosorbent Assay, Control

Journal: Nanomaterials

Article Title: Scavenger Receptor A1 Mediates the Uptake of Carboxylated and Pristine Multi-Walled Carbon Nanotubes Coated with Bovine Serum Albumin

doi: 10.3390/nano11020539

Figure Lengend Snippet: Immunofluorescence microscopy and flow cytometric analysis of WT and SR-A1 knockout RAW 264.7 cells. Immunofluorescence images of (A) control WT RAW 264.7 cells without mouse anti-SR-AI/MSR Alexa Fluor ® 488-conjugated antibody, (B) WT RAW 264.7 cells incubated with mouse anti SR-AI/MSR Alexa Fluor ® 488-conjugated antibody, and ( C , D ) two different clones (C4 and B11) of SR-A1 knockout RAW 264.7 cells incubated with anti-mouse SR-AI/MSR Alexa Fluor ® 488-conjugated antibody. Hoechst 33342 staining is shown in blue and Alexa Fluor ® 488 staining is shown in green. All images are normalized to the same intensity scale, and the scale bars represent 20 μm. (E) Flow cytometry analyses where the black line represents WT RAW 264.7 cells incubated with the rat IgG2B Alexa Fluor ® 488-conjugated monoclonal antibody as the isotype control, the red line represents WT RAW 264.7 cells incubated with mouse anti-SR-AI/MSR Alexa Fluor ® 488-conjugated antibody, and the green and purple lines represent two different clones (C4 and B11, respectively) of SR-A1 knockout RAW 264.7 cells incubated with mouse anti-SR-AI/MSR Alexa Fluor ® 488-conjugated antibody. The x-axis denotes fluorescence detected in the 518–548 nm spectral region, and the y-axis denotes the number of events for each analysis.

Article Snippet: The cells were stained with 5 μg mouse SR-AI/MSR Alexa Fluor ® 488-conjugated antibody (R&D Systems cat. No. FAB1797G) or a rat IgG2B Alexa Fluor ® 488-conjugated monoclonal antibody (R&D Systems cat. No. IC013G) as the isotype control for 30 min at 4 °C in the dark.

Techniques: Immunofluorescence, Microscopy, Knock-Out, Control, Incubation, Clone Assay, Staining, Flow Cytometry, Fluorescence