Journal: Frontiers in Medicine

Article Title: Gene Expression in Amnion-Derived Cells Cultured on Recombinant Laminin 332—A Preliminary Study

doi: 10.3389/fmed.2021.719899

Figure Lengend Snippet: Antibodies and isotype controls used for immunodetection of integrin subunits and CK-7.

Article Snippet: ITGB4 , Anti-integrin beta 4 antibody, clone 439-9B (Novus Biologicals) , Alexa Fluor 488 Mouse monoclonal Anti-Rat IgG2b heavy chain (Abcam) , Rat IgG2b Isotype Control (Novus Biologicals).

Techniques: Immunodetection, Control, Purification, Plasmid Preparation

Journal: Nanomaterials

Article Title: Scavenger Receptor A1 Mediates the Uptake of Carboxylated and Pristine Multi-Walled Carbon Nanotubes Coated with Bovine Serum Albumin

doi: 10.3390/nano11020539

Figure Lengend Snippet: Immunofluorescence microscopy and flow cytometric analysis of WT and SR-A1 knockout RAW 264.7 cells. Immunofluorescence images of (A) control WT RAW 264.7 cells without mouse anti-SR-AI/MSR Alexa Fluor ® 488-conjugated antibody, (B) WT RAW 264.7 cells incubated with mouse anti SR-AI/MSR Alexa Fluor ® 488-conjugated antibody, and ( C , D ) two different clones (C4 and B11) of SR-A1 knockout RAW 264.7 cells incubated with anti-mouse SR-AI/MSR Alexa Fluor ® 488-conjugated antibody. Hoechst 33342 staining is shown in blue and Alexa Fluor ® 488 staining is shown in green. All images are normalized to the same intensity scale, and the scale bars represent 20 μm. (E) Flow cytometry analyses where the black line represents WT RAW 264.7 cells incubated with the rat IgG2B Alexa Fluor ® 488-conjugated monoclonal antibody as the isotype control, the red line represents WT RAW 264.7 cells incubated with mouse anti-SR-AI/MSR Alexa Fluor ® 488-conjugated antibody, and the green and purple lines represent two different clones (C4 and B11, respectively) of SR-A1 knockout RAW 264.7 cells incubated with mouse anti-SR-AI/MSR Alexa Fluor ® 488-conjugated antibody. The x-axis denotes fluorescence detected in the 518–548 nm spectral region, and the y-axis denotes the number of events for each analysis.

Article Snippet: The cells were stained with 5 μg mouse SR-AI/MSR Alexa Fluor ® 488-conjugated antibody (R&D Systems cat. No. FAB1797G) or a rat IgG2B Alexa Fluor ® 488-conjugated monoclonal antibody (R&D Systems cat. No. IC013G) as the isotype control for 30 min at 4 °C in the dark.

Techniques: Immunofluorescence, Microscopy, Knock-Out, Control, Incubation, Clone Assay, Staining, Flow Cytometry, Fluorescence